Determination of S alleles in Paviot × Levent apricot progenies by PCR and controlled pollination
Keywords:Apricot, Paviot, Levent, self-incompatibility, PCR
In this study, the sexual incompatibility of Paviot and Levent apricot parents and 89 F1 (Paviot × Levent) progenies was determined by self-pollination experiments and S-allele-specific polymerase chain reaction (PCR) technique. According to the self-pollination and isolation analyses under field conditions, it was found that the Paviot genotype is self-compatible (SC), whereas the Levent genotype is self-incompatible (SI). It was determined that, of all the progenies, 55 had a fruit set below 5% and were self-incompatible, whereas 34 had a fruit set over 5% and were self-compatible. The PCR-based techniques showed that, in parallel to the data obtained from the field studies, 55 F1 progenies did not have Sc allele, whereas 34 progenies involved Sc allele. There were ScS2 alleles in the Paviot genotype and S20S52 alleles in the Levent genotype. It was determined that there were S2S20, S2S52, ScS20, and ScS52 alleles in 89 F1 progenies and the distribution of the four alleles in the progenies was found to be as follows: 35.9% S2S20, 25.8% S2S52, 23.6% ScS20, and 14.6% ScS52. F1 progenies nos. 41, 46, 86, and 89 should be used as pollinators in further breeding studies.
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