<i>Laserpitium ochridanum</i>: antioxidant, antimicrobial and anti-quorum sensing activities against <i>Pseudomonas aeruginosa</i>


  • Ksenija Slave Mileski University of Belgrade, Faculty of Biology Institute of Botany and Botanical Garden “Jevremovac” Studentski trg 16, 11000 Belgrade, Serbia
  • Ana D Ćirić
  • Jovana D. Petrović Department of Plant Physiology, Institute for Biological Research “Siniša Stanković“, University of Belgrade
  • Mihailo S Ristić Institute for Medicinal Plant Research "Dr. Josif Pančić", Belgrade, Serbia
  • Vlado S Matevski Department of Biology, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University, Institute for Biology and Macedonian Academy of Sciences and Arts, Skopje, Macedonia
  • Petar D Marin Department of Morphology and Systematic of Plants, Institute of Botany and Botanicalgarden “Jevremovac”, Faculty of Biology, University of Belgrade
  • Ana M. Džamić




Laserpitium ochridanum, Essential oil, Antioxidant activity, Antimicrobial activity, Anti–quorum sensing activity


This study shows Laserpitium ochridanum essential oil composition, its antifungal potency, and possible antioxidant, antimicrobial and anti-quorum sensing activities of different extracts. Monoterpene hydrocarbons (40.9%) found to be the most abundant group of constituents in the oil. Sabinene (22.8%), viridiflorol (14.7%) and α-pinene (11.40%) were the main compounds of the oil. Ethanolic extract had the highest antioxidant capacity in DPPH and ABTS assays and was the richest in polyphenols. Microdilution method revealed the strongest antibacterial activity of ethanolic extracts in comparison to other tested extracts and streptomycin. L. ochridanum oil evidenced the best antifungal potential against used micromycetes. Results of anti-quorum sensing activity assay indicated high affection of aqueous extract in reduction of PAO1 pyocianin production (18.07%).Used samples possessed slight reduction of twitching and swimming mobility. Excellent antifungal oil activity, good antioxidant, antimicrobial and anti-quorum activity of L. ochridanum extracts was found.  This study shows for the first time anti-guorum sensing activity against Pseudomonas aeruginosa of L. ochridanum, as well as its significant antioxidant potential. This study was conducted due to adventives of usage of natural products as efficient substitution for synthetic agents in treatment of various medical conditions.

Author Biography

Ana D Ćirić

Department of Plant Physiology, Institute for Biological Research “Siniša Stanković“, University of Belgrade