Detection of the methylated Trichothecenegenes Tri5 and Tri14 in <em>Fusarium sporotrichioides</em> using the endonuclease <em>Msp</em>JI during host plant infections

Authors

  • Frank Niepold Julius Kühn-Institut (JKI) – Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Braunschweig

DOI:

https://doi.org/10.5073/JfK.2012.02.02

Keywords:

Restriction endonuclease MspJI, Fusarium- DNA-methylation, PCR, host plant infection

Abstract

A survey was made for Fusarium sporotrichioides on its methylation of DNA by choosing the mycotoxin genes Tri5 and Tri14 and applying different temperature and infection conditions. The restriction endonuclease MspJI was used which only digests DNA when the nucleotide sequence Guanosin/Cytosine (G/C) is methylated. Unmethylated G/C-DNA is not restricted by this enzyme and therefore a differentiation is possible in a simple way. A successful restriction and non-restriction was proved by using PCR-primer, covering both genes. Methylation of the coding DNA for the two mycotoxin genes Tri5 und Tri14 was detected by MspJI, only when F. sporotrichioides infected its host plants barley and maize. All other conditions did not have any influence on the methylation of the two genes.

 

 

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Published

2012-02-01

Issue

Vol. 64 No. 2 (2012)

Section

Original Article

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