Searching for biomarkers in the grape leaf metabolome

Authors

  • Maike Elfert Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen, Institut für ökologische Chemie, Pflanzenanalytik und Vorratsschutz, Quedlinburg
  • Detlef Ulrich Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen, Institut für ökologische Chemie, Pflanzenanalytik und Vorratsschutz, Quedlinburg
  • Michael Fischer Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen, Institut für Pflanzenschutz in Obst- und Weinbau, Siebeldingen
  • Christoph Hoffmann Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen, Institut für Pflanzenschutz in Obst- und Weinbau, Siebeldingen
  • Thomas Strumpf Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen, Institut für ökologische Chemie, Pflanzenanalytik und Vorratsschutz, Berlin-Dahlem

DOI:

https://doi.org/10.5073/JfK.2013.01.03

Keywords:

Organic viticulture, non-targeted analysis, SPME-GC-MS, grapevine leaves, resistance biomarker, powdery and downy mildew

Abstract

Powdery and downy mildew of grapevine are widespread diseases, which may cause severe harvest losses. In organic farming only copper containing fungicides are permitted. In relation to the longtime application of copper containing plant protection products the impact of copper deposit in soil and its effect to soil organisms are discussed nationally and internationally. Therefore, developing alternative plant protection strategies is an important field of research. Thus, the metabolite profiles of resistant grapevine species (American wild type species and old hybrid grapevine varieties) are compared during a triennial survey with representatives of the more susceptible Euro­pean grapevine cultivars by non-targeted analysis assays. This might give some hints to so far unknown resistance biomarkers or elicitors. The different volatile organic compounds (VOCs) of the leaf metabolome were analyzed by headspace-SPME-GC-MS. In an annual survey 81 out of 120 volatiles detected in grapevine leaves were identified. Though many of the components are included in several individual species and varieties, there are detectable differences with respect to their quality and quantity. Multivariate statistical data processing (PCA) showed that both the leaf development stadium and the grapevine species (resp. variety) have a great impact on the volatile profile. In contrast, the distinctions in the metabolomes of different plants which belong to the same variety are less obvious.

 

 

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Published

2013-01-01

Issue

Vol. 65 No. 1 (2013)

Section

Original Article

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