Detection and molecular analysis of <em>Hop latent virus</em> and <em>Hop latent viroid</em> in hop samples from Poland

Authors

  • Angelika Ziegler Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut – Federal Research Centre for Cultivated Plants, Quedlinburg, Germany
  • Magdalena Kawka Institute of Soil Science and Plant Cultivation-State Research Institute, Department of Plant Breeding and Biotechnology, Pulawy, Poland
  • Marcin Przybys Institute of Soil Science and Plant Cultivation-State Research Institute, Department of Plant Breeding and Biotechnology, Pulawy, Poland
  • Teresa Doroszewska Institute of Soil Science and Plant Cultivation-State Research Institute, Department of Plant Breeding and Biotechnology, Pulawy, Poland
  • Urszula Skomra Institute of Soil Science and Plant Cultivation-State Research Institute, Department of Plant Breeding and Biotechnology, Pulawy, Poland
  • Ute Kastirr Institute for Epidemiology and Pathogen Diagnostics, Julius Kühn-Institut – Federal Research Centre for Cultivated Plants, Quedlinburg, Germany
  • Jaroslav Matoušek Biol. Centre AS CR v.v.i., Institute of Plant Molecular Biology, 37005 České Budějovice, Czech Republic
  • Jörg Schubert Institute for Biosafety in Plant Biotechnology, Julius Kühn-Institut – Federal Research Centre for Cultivated Plants, Quedlinburg, Germany

Keywords:

Hop latent virus, hop latent viroid, hop stunt viroid, RT-PCR, real time RT-PCR, ELISA

Abstract

Monitoring the occurrence of virus diseases in plants is important for the implementation of early control measures and prevention of further disease spread. In Poland, in 2004 a health programme for hop was started to eliminate viruses and viroids. In 2012/13, in vitro plants, samples from the IUNG-PIB experimental station and commercial hop gardens in Poland were tested for Hop latent virus (HpLV), and Hop latent and Hop stunt viroids (HpLVd and HpSVd). For virus testing, RT-PCR and ELISA methods were used. In order to detect hop viroids, RT-PCR was employed. The overall incidence of HpLV and hop viroids was lower than reported before the start of the programme. Cloning and sequencing revealed that the HpLV and the HpLVd from Polish sources are very similar to the type sequences and the Czech sources.

DOI: 10.5073/JfK.2014.07.04, https://doi.org/10.5073/JfK.2014.07.04

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Published

2014-07-01

Issue

Vol. 66 No. 7 (2014)

Section

Original Article

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