A simple and fast light-microscopical method to identify rust species (<em>Puccinia</em> spp.) on wheat (<em>Triticum aestivum</em>) using germ pore features of urediniospores

Authors

  • Ramona Buchheit Staatliches Museum für Naturkunde Karlsruhe, Erbprinzenstraße 13, 76133 Karlsruhe
  • Kamilla Zegermacher Landwirtschaftliches Technologiezentrum Augustenberg, Neßlerstraße 25, 76227 Karlsruhe
  • Gabriele Zgraja Landwirtschaftliches Technologiezentrum Augustenberg, Neßlerstraße 25, 76227 Karlsruhe
  • Jan Hinrichs-Berger Landwirtschaftliches Technologiezentrum Augustenberg, Neßlerstraße 25, 76227 Karlsruhe
  • Markus Scholler Staatliches Museum für Naturkunde Karlsruhe, Erbprinzenstraße 13, 76133 Karlsruhe

DOI:

https://doi.org/10.5073/JfK.2020.02-03.01

Keywords:

Pucciniales, Puccinia graminis, P. triticina, P. striiformis, phase contrast microscopy, germ pore features, species identification, sequence analyses, method comparison

Abstract

In Central Europe there are three wheat rusts (Pucciniales) all of which are economically important. These are the (black) stem rust or wheat stem rust (Puccinia graminis), the leaf rust (P. triticina) and the stripe rust (P. striiformis). In order to monitore or control them effec­tively, efficient and reliable identification methods are needed. Here, a simple light-microscopical (phase contrast) method using germ pore features of urediniospores for identification is presented. This method makes it possible to identify the rust species exactly. In the present study this method is compared with the ITS sequence analyses with respect to identification accuracy, time and material costs.

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Published

2020-03-01

Issue

Vol. 72 No. 2-3 (2020)

Section

Original Article

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