The <em>in vitro</em> cultivation of <em>Bursaphelenchus</em> spp. at the reference laboratory for quarantine pests at Julius Kühn-Institut in Braunschweig

Authors

  • Björn Hoppe Julius Kühn-Institut (JKI) – Bundesforschungsinstitut für Kulturpflanzen, Institut für nationale und internationale Angelegenheiten der Pflanzengesundheit, Braunschweig
  • Helen Braasch Julius Kühn-Institut (JKI) – Bundesforschungsinstitut für Kulturpflanzen, Institut für nationale und internationale Angelegenheiten der Pflanzengesundheit, 14532 Kleinmachnow; * 14471 Potsdam, Kantstr. 5 (im Ruhestand)
  • Silvia Urban Julius Kühn-Institut (JKI) – Bundesforschungsinstitut für Kulturpflanzen, Institut für nationale und internationale Angelegenheiten der Pflanzengesundheit, Braunschweig
  • Thomas Schröder BMEL – Bundesministerium für Ernährung und Landwirtschaft, Bonn

DOI:

https://doi.org/10.5073/JfK.2020.08.12

Keywords:

Bursaphelenchus xylophilus, in vitro-breeding

Abstract

The Forest quarantine laboratory at Julius Kühn-Institut in Braunschweig (Germany) curates a unique collection of living Bursaphelenchus species, permanent slides and ITS-RFLP profiles. The collection was initiated by Dr. Helen Braasch and currently comprises 48 species in 308 different isolates. These isolates were collected over 30 years across the globe from various habitats and sources, like forest trees and wood imports including wooden packaging material. Cultivation of the nematodes on sporulating and non-sporulating Botrytis cinerea is sophisticated, labor-intensive and requires both, experience and patience.

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Published

2020-08-01

Issue

Vol. 72 No. 8 (2020): Special issue Plant health

Section

Review

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