A rapid method to monitor parasitoid infestations in Drosophila suzukii populations

Authors

  • Jan Dudzic Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Dossenheim, Germany. https://orcid.org/0000-0002-1657-6958
  • Jakob Martin Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Biological Control, Dossenheim, Germany.
  • Annette Herz Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Biological Control, Dossenheim, Germany.
  • Astrid Eben Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Dossenheim, Germany.

DOI:

https://doi.org/10.5073/JfK.2023.09-10.05

Keywords:

Drosophila suzukii, parasitoids, Spotted wing Drosophila, monitoring

Abstract

Parasitoid wasps are efficient natural enemies of other insects. They are in focus as an important biological pest management element to control invasive pest species. While spreading into new areas these invasive pests are often followed by their natural parasitoids from their home regions. The monitoring of native and adventive parasitoids, and their success in controlling the pest populations in the field, is cumbersome and relies mainly on hatching experiments. Here, we present a rapid method to detect parasitoid infestations in the invasive agricultural pest Drosophila suzukii. We specifically amplified a hymenopteran 28S rRNA sequence from DNA or RNA extracted from fly pupae. This allowed the determination of parasitoid infestations at the species level.

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Published

2023-10-05

Issue

Section

Short Communication