NIRS for vicine and convicine content of faba bean seed allowed GWAS to prepare for marker-assisted adjustment of seed quality of German winter faba beans

Authors

  • Winda Puspitasari Georg-August-Universität Göttingen, Department of Crop Sciences, Division of Plant Breeding Methodology, Göttingen, Germany. Current address: National Nuclear Energy Agency, Center for Application of Isotope and Radiation, Jakarta, Indonesia.
  • Björn Allemann Georg-August-Universität Göttingen, Department of Crop Sciences, Division of Plant Breeding Methodology, Göttingen, Germany. Current address: Rodetaler Naturhof, Rodetal, Bovenden, Germany.
  • Deepti Angra University of Reading, School of Agriculture, Policy and Development, Reading, UK.
  • Helen Appleyard Analytical Services, National Institute of Agricultural Botany, Cambridge, UK.
  • Wolfgang Ecke Georg-August-Universität Göttingen, Department of Crop Sciences, Division of Plant Breeding Methodology, Göttingen, Germany.
  • Christian Möllers Georg-August-Universität Göttingen, Department of Crop Sciences, Division of Crop Plant Genetics, Germany.
  • Tanja Nolte Georg-August-Universität Göttingen, Department of Crop Sciences, Division of Animal Breeding and Genetics, Göttingen, Germany.
  • Randy W. Purves University of Saskatchewan, Department of Plant Sciences, Saskatoon, Canada. Current address: Canadian Food Inspection Agency, Centre for Veterinary Drug Residues, Saskatoon, Canada.
  • Carsten Renner Georg-August-Universität Göttingen, Department of Crop Sciences, Division of Plant Breeding Methodology, Göttingen, Germany. Current address: Landwirtschaftskammer Nordrhein-Westfalen. Fachschule für Agrarwirtschaft, Meschede, Germany.
  • Thomas Robertson-Shersby-Harvie University of Reading, School of Agriculture, Policy and Development, Reading, UK.
  • Rebecca Tacke Georg-August-Universität Göttingen, Department of Crop Sciences, Division of Plant Breeding Methodology, Göttingen, Germany.
  • Alex Windhorst Georg-August-Universität Göttingen, Department of Crop Sciences, Division of Plant Breeding Methodology, Göttingen, Germany.
  • Sonja Yaman Georg-August-Universität Göttingen, Department of Crop Sciences, Division of Plant Breeding Methodology, Göttingen, Germany.
  • Wolfgang Link Georg-August-Universität Göttingen, Department of Crop Sciences, Division of Plant Breeding Methodology, Göttingen, Germany.

DOI:

https://doi.org/10.5073/JfK.2022.01-02.01

Keywords:

Faba bean, Vicia faba L., Vicine, Convicine, NIRS, GWAS, QTL, breeding research

Abstract

GWAS was applied to the antinutritive compounds vicine and convicine (V, C) in winter faba bean. V, C and V + C data for 189 inbred lines (five environments) were predicted by NIRS. These lines do not carry the strong “vc-“ allele (locus VC1). Lab data for 646 samples enabled our NIRS calibration, which performed well for V and V + C yet poor for C. Heritability was high (0.911; 0.868) for V and V + C and lower for C (0.737). From the 2542 mapped SNPs, 47 were significantly associated with V and one with V + C. Four SNPs mapped near to the VC1 locus and were significant for V. Seemingly, non-“vc-“ alleles at that locus contributed to V variation. Marker-assisted breeding with this germplasm can reduce the V + C content to about 0.44%, compared to the current lowest line with 0.55%. Further research will show inasmuch this can serve agronomy and breeding.

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Published

2022-01-21

Issue

Vol. 74 No. 01-02 (2022)

Section

Original Article

License

Copyright (c) 2022 Winda Puspitasari, Björn Allemann, Deepti Angra, Helen Appleyard, Wolfgang Ecke, Christian Möllers, Tanja Nolte, Randy W. Purves, Carsten Renner, Thomas Robertson-Shersby-Harvie, Rebecca Tacke, Alex Windhorst, Sonja Yaman, Wolfgang Link

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